S-(2,4-Dinitrophenyl)-Glutathione is primarily used in biochemical research as a substrate or reference compound for studying the activity of glutathione S-transferase (GST) enzymes. These enzymes play a critical role in detoxification processes within cells by catalyzing the conjugation of glutathione to various electrophilic compounds. By utilizing this chemical, researchers can measure GST activity, assess enzyme kinetics, and evaluate the efficiency of detoxification pathways. It is also employed in assays to screen for potential inhibitors or activators of GST, which can have implications in drug development and toxicology studies. Additionally, it serves as a tool in understanding the mechanisms of cellular defense against oxidative stress and chemical toxicity.