SAG hydrochloride is widely used in biochemical research, particularly in studies involving the Hedgehog signaling pathway. It acts as a potent agonist of the Smoothened receptor, which is a key component in this pathway. Researchers utilize it to activate the pathway in various experimental models, helping to understand its role in cell differentiation, proliferation, and tissue regeneration. Additionally, it is employed in drug discovery and development to screen for potential inhibitors or modulators of the Hedgehog pathway, which is implicated in several cancers and developmental disorders. Its application extends to studying embryonic development and regenerative medicine, where the pathway plays a critical role.