Smoothened Agonist (SAG) HCl is widely used in research to activate the Hedgehog signaling pathway, which plays a critical role in cell differentiation, proliferation, and tissue regeneration. It is particularly valuable in studies involving stem cell biology, as it helps induce the differentiation of stem cells into specific lineages, such as neurons or muscle cells. In cancer research, SAG HCl is utilized to investigate the role of Hedgehog signaling in tumor growth and metastasis, providing insights into potential therapeutic targets. Additionally, it is employed in developmental biology to study embryonic patterning and organogenesis. Its ability to modulate the Hedgehog pathway makes it a key tool in drug discovery and the development of treatments for diseases linked to pathway dysregulation.