Purmorphamine is widely used in biomedical research due to its ability to activate the Hedgehog signaling pathway, which plays a critical role in cell differentiation and tissue development. It is particularly valuable in stem cell studies, where it promotes the differentiation of mesenchymal stem cells into osteoblasts, making it a key tool in bone regeneration research. Additionally, purmorphamine is utilized in cancer research to investigate the role of Hedgehog signaling in tumor growth and progression. Its applications extend to drug discovery, where it serves as a reference compound for developing therapies targeting developmental disorders and cancers linked to this pathway.