JW67 is primarily utilized in scientific research, particularly in the field of biology and pharmacology. It is known for its role as a selective inhibitor of the Hedgehog signaling pathway, which is crucial in the study of cellular development and cancer biology. Researchers use JW67 to investigate the effects of inhibiting this pathway on various cellular processes, including cell proliferation, differentiation, and tumor growth. Its application aids in understanding the molecular mechanisms underlying certain cancers and in the development of potential therapeutic strategies targeting the Hedgehog pathway.