Used as a heterobifunctional crosslinking agent in bioconjugation, particularly for coupling peptides or proteins to other biomolecules or surfaces. The bromoacetamido group reacts selectively with thiol groups (–SH) in cysteine residues, forming stable thioether bonds, while the activated ester (N-hydroxysuccinimide-like) end reacts efficiently with primary amines (–NH₂) in lysine residues or terminal amino groups. This dual reactivity enables controlled, site-specific conjugation in the development of antibody-drug conjugates (ADCs), diagnostic probes, and immobilization of biomolecules on solid supports. Its design allows for defined linker spacing and improved conjugation efficiency in aqueous or mixed solvent systems.