Dichloroglyoxime is primarily used in analytical chemistry as a reagent for the detection and quantification of heavy metals such as nickel and cobalt. It forms intensely colored complexes with these metals, allowing for accurate spectrophotometric analysis. Additionally, it serves as a versatile reagent in organic synthesis for the formation of oximes, heterocyclic compounds, and nitrogen-containing molecules, which are important intermediates in pharmaceuticals and agrochemicals. It also acts as a precursor in the synthesis of metal coordination complexes studied for catalytic properties, making it valuable in research and industrial chemistry for developing new materials and processes.