Used in histology and pathology to detect the presence of inorganic phosphate deposits in tissues, particularly in bone, teeth, and calcified lesions. The kit helps visualize mineralization in samples by staining calcium phosphate salts black or dark brown, allowing researchers to study conditions like ectopic calcification, atherosclerosis, and bone disorders. Commonly applied in tissue sections from paraffin-embedded or frozen samples. Also used in research on kidney disease, vascular calcification, and biomaterials to assess mineral deposition. The stain is especially valuable in distinguishing calcium from other pigments in pathological specimens.