Orange G is widely used as a biological stain in microscopy, particularly for highlighting cellular structures in tissue samples. It is often employed in histology to stain collagen and cytoplasm, providing contrast in microscopic analysis. Additionally, it is utilized in protein electrophoresis as a tracking dye to monitor the progress of protein separation. In the textile industry, it serves as a dye for coloring fabrics, especially silk and wool, due to its vibrant orange hue. It is also used in the food industry as a coloring agent for certain products, though its use in food is less common due to regulatory restrictions.