Used as a biological stain in microscopy to identify lipids and lipoproteins in tissues. It helps in distinguishing between neutral fats and fatty acids. Also employed in histology for staining mitochondria and other cellular components. Applied in fluorescence microscopy due to its ability to emit bright red fluorescence under specific conditions. Utilized in research to study cell membranes and lipid distribution. Sometimes used in diagnostic procedures to detect certain types of cancers by highlighting abnormal lipid accumulation.