Aniline Blue, sodium salt, is widely used in biological staining techniques, particularly in histology and microscopy. It is a vital dye for highlighting collagen fibers in tissue samples, allowing researchers and pathologists to distinguish between different tissue structures under a microscope. The dye binds specifically to collagen, providing a clear contrast that aids in the study of connective tissues and their abnormalities. In addition to its use in collagen staining, Aniline Blue is employed in plant biology to study pollen viability and cell wall components. It helps in identifying and analyzing pollen tube growth and other cellular processes in plants. The dye’s ability to selectively stain certain cellular structures makes it a valuable tool in both medical and botanical research.