Safranine T is widely used as a biological stain in microscopy, particularly for highlighting cell nuclei and chromosomes in plant and animal tissues. It is also employed in histology to differentiate between various tissue structures. In the textile industry, Safranine T serves as a dye for silk, cotton, and leather, providing vibrant red or pink shades. Additionally, it is utilized in analytical chemistry as an indicator for redox titrations due to its distinct color change properties. In microbiology, it aids in the identification of certain bacteria by staining specific cellular components. Safranine T is also used in the preparation of light-sensitive materials for photographic applications.