Orange G is widely used as a biological stain in histology and cytology for highlighting specific structures in tissue samples. It is particularly effective in staining collagen fibers, making it valuable in connective tissue studies. In microbiology, it serves as a dye for visualizing microorganisms under a microscope. Additionally, Orange G is employed in electrophoresis techniques to track protein migration due to its distinct color. It is also utilized in the textile industry for dyeing fabrics, providing vibrant orange hues. In some cases, it is used as a pH indicator in laboratory experiments, changing color in response to acidity or alkalinity.