Used primarily in histology and microbiology for staining fungal elements and certain bacteria in tissue sections. It enhances the visibility of fungal cell walls, making it valuable in diagnosing systemic fungal infections such as aspergillosis, mucormycosis, and candidiasis. The stain imparts a black or dark brown color to fungi against a green or light background, allowing for high contrast under the microscope. It is especially effective in detecting thin or delicate hyphae that may be missed with other staining methods. Commonly used in clinical pathology and research settings where precise identification of microbial structures in paraffin-embedded tissues is required.