Used primarily in histology and pathology, this staining solution is applied to differentiate fibrin and other tissue components in microscopic examination. It selectively stains fibrin bright yellow-orange under fluorescence microscopy, making it highly effective for identifying microthrombi in blood vessels and assessing clot formation in tissue sections. The contrast provided by the stain allows clear distinction between fibrin and collagen, which is crucial in diagnosing conditions such as disseminated intravascular coagulation (DIC) and other thrombotic disorders. It is often used in combination with other stains to enhance specificity and improve diagnostic accuracy in tissue analysis.