Orange I is primarily used as a dye in various industries due to its vibrant color. It is commonly applied in the textile industry for dyeing fabrics, providing a bright and long-lasting orange hue. In biological and chemical laboratories, Orange I serves as a pH indicator with a visual transition interval from orange at pH 7.6 to red at pH 8.9, aiding in experiments and analyses. Its ability to bind with proteins also makes it useful in staining techniques for microscopy, helping to visualize cellular structures.