UNC0224 is primarily utilized in biochemical research as a potent and selective inhibitor of the G9a histone methyltransferase enzyme. Its application is significant in studying epigenetic regulation, as G9a plays a crucial role in histone methylation, which influences gene expression and chromatin structure. Researchers use UNC0224 to investigate the impact of G9a inhibition on cellular processes such as differentiation, proliferation, and apoptosis. This compound is also employed in cancer research to explore its potential in reversing aberrant epigenetic modifications associated with tumorigenesis. Additionally, UNC0224 aids in understanding the therapeutic implications of targeting histone methyltransferases in diseases linked to epigenetic dysregulation.