GW4064 is primarily used in research settings as a potent and selective agonist for the farnesoid X receptor (FXR), a nuclear receptor involved in regulating bile acid, lipid, and glucose metabolism. It is widely employed in studies investigating the role of FXR in metabolic disorders, such as non-alcoholic fatty liver disease (NAFLD), cholesterol homeostasis, and insulin resistance. Researchers utilize GW4064 to explore its potential therapeutic effects in modulating bile acid synthesis and improving metabolic parameters. Additionally, it serves as a valuable tool in understanding the molecular mechanisms of FXR signaling pathways and its impact on inflammation and fibrosis in liver-related diseases. Its application extends to drug discovery efforts aimed at developing FXR-targeted therapies for metabolic and hepatic conditions.