Lacto-N-difucohexaose II is primarily utilized in the field of nutritional science and infant formula development. It serves as a key component in mimicking the complex oligosaccharides found in human breast milk, which are crucial for promoting healthy gut microbiota in infants. This compound aids in enhancing the immune system by acting as a prebiotic, supporting the growth of beneficial bacteria such as Bifidobacteria. Additionally, it plays a role in protecting against pathogens by preventing their adhesion to the intestinal lining, thereby reducing the risk of infections. Its application extends to research focused on understanding the functional benefits of human milk oligosaccharides and developing advanced nutritional products for infants who cannot be breastfed.