Lacto-N-neotetraose is primarily utilized in the field of infant nutrition due to its role as a human milk oligosaccharide (HMO). It serves as a prebiotic, promoting the growth of beneficial gut bacteria in infants, which is crucial for developing a healthy microbiome. This compound also plays a significant role in enhancing the immune system by preventing the adhesion of harmful pathogens to the gut lining, thereby reducing the risk of infections. Additionally, it is being researched for its potential applications in dietary supplements aimed at improving gut health in adults. Its inclusion in infant formulas aims to closely mimic the composition and benefits of human breast milk, supporting overall infant health and development.