2-Thiohydantoin is widely used in organic synthesis as a building block for the preparation of various heterocyclic compounds. It serves as a key intermediate in the production of pharmaceuticals, particularly in the development of drugs targeting neurological disorders and cancer. Its derivatives are explored for their potential antimicrobial and antioxidant properties, making it valuable in medicinal chemistry. Additionally, 2-thiohydantoin is utilized in the synthesis of peptides and peptidomimetics, which are essential in biochemical research and drug discovery. Its role in the development of novel therapeutic agents highlights its significance in advancing healthcare solutions.