O-Acetyl Salicylhydroxamic Acid is primarily used in the field of analytical chemistry as a chelating agent. It forms stable complexes with various metal ions, making it valuable for the separation and determination of metals in different samples. This compound is particularly effective in spectrophotometric analysis, where it helps in the quantification of trace metals by forming colored complexes that can be easily measured.

In addition to its analytical applications, it is also explored in medicinal chemistry for its potential biological activities. Research has indicated that derivatives of hydroxamic acids, including O-Acetyl Salicylhydroxamic Acid, may exhibit antimicrobial and anticancer properties. These properties are attributed to their ability to inhibit enzymes such as histone deacetylases (HDACs), which play a crucial role in cell proliferation and differentiation.

Furthermore, it is utilized in organic synthesis as an intermediate for the preparation of more complex hydroxamic acid derivatives. These derivatives are often investigated for their pharmacological activities and potential use in drug development. Its role in synthesizing novel compounds with therapeutic potential makes it a compound of interest in pharmaceutical research.