Used in immunological research to quantify levels of Tim-3, a protein involved in regulating T cell responses. Helps study immune checkpoint pathways, particularly in contexts like chronic infection, autoimmunity, and cancer. Enables researchers to assess Tim-3 expression in cell cultures or biological samples, supporting investigation into immune exhaustion and therapeutic interventions targeting the Tim-3 pathway. Widely applied in preclinical studies for drug development and biomarker discovery.