Biotinyl tyramide is widely used in molecular biology and immunohistochemistry for signal amplification in detection assays. It plays a crucial role in tyramide signal amplification (TSA) techniques, where it enhances the sensitivity of detecting low-abundance proteins, nucleic acids, or other biomolecules. This compound is particularly valuable in fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) to visualize and localize specific targets within cells or tissues. By leveraging the high affinity of biotin for streptavidin, biotinyl tyramide allows for the binding of multiple reporter molecules, significantly boosting the signal intensity. This makes it an essential tool for researchers studying gene expression, protein localization, and cellular processes with high precision and clarity.