ELISA kit is widely used in medical and biological research for detecting and quantifying substances such as peptides, proteins, antibodies, and hormones. It is commonly applied in disease diagnosis, including infectious diseases like HIV, hepatitis, and Lyme disease, as well as autoimmune disorders and cancer biomarkers. The kit enables high-throughput screening in clinical laboratories and research settings due to its sensitivity and specificity. It is also used in food industry testing for allergens and in pharmaceutical development to monitor immune responses during drug trials.