ELISA kit is widely used in medical and biological research for detecting and quantifying substances such as peptides, proteins, antibodies, and hormones. It is commonly applied in disease diagnosis, including infectious diseases like HIV, hepatitis, and more recently, SARS-CoV-2. The kit enables high sensitivity and specificity in measuring target molecules in complex samples such as serum, plasma, or cell culture supernatants. It is also used in food safety testing to detect allergens or contaminants, and in pharmaceutical development for monitoring immune responses during clinical trials. Its versatility and reliability make it a standard tool in clinical laboratories and research settings.