ELISA kit is widely used in medical and biological research for detecting and quantifying substances such as peptides, proteins, antibodies, and hormones. It is commonly applied in disease diagnosis, including infectious diseases like HIV, hepatitis, and more recently, SARS-CoV-2. The kit enables high-throughput screening in clinical laboratories and research settings due to its sensitivity and specificity. It is also used in food industry testing for allergens and in quality control of biopharmaceuticals. The assay format allows measurement in serum, plasma, cell culture supernatants, and other biological samples, making it a versatile tool in both diagnostics and research.