ELISA kits are widely used in medical and biological research for detecting and quantifying substances such as peptides, proteins, antibodies, and hormones. They are essential in diagnosing infectious diseases like HIV, hepatitis, and Lyme disease by identifying specific antigens or antibodies in blood samples. In clinical laboratories, ELISA kits provide a reliable method for screening patient samples due to their high sensitivity and specificity. They are also used in food industry testing to detect allergens such as peanuts or gluten. In research settings, these kits help measure cytokine levels, monitor immune responses, and validate biomarkers. Their ease of use, scalability, and compatibility with high-throughput systems make them a standard tool in both diagnostics and life science research.