ELISA kit is widely used in medical and biological research for detecting and quantifying substances such as peptides, proteins, antibodies, and hormones. It is commonly applied in disease diagnosis, including infectious diseases like HIV, hepatitis, and more recently, SARS-CoV-2. The kit enables high sensitivity and specificity in measuring analytes in complex biological samples such as serum, plasma, and cell culture supernatants. It is also used in food safety testing to detect allergens or contaminants, and in pharmaceutical development for monitoring immune responses during clinical trials. Due to its reliability and scalability, ELISA kits are standard tools in both clinical laboratories and research settings.