ELISA kit is widely used in medical and biological research for detecting and quantifying substances such as peptides, proteins, antibodies, and hormones. It is commonly applied in disease diagnosis, including infectious diseases like HIV, hepatitis, and more recently, SARS-CoV-2. The kit enables high sensitivity and specificity in measuring analytes in complex biological samples such as serum, plasma, and cell culture supernatants. It is also used in food safety testing to detect allergens or contaminants and in environmental science to monitor pollutants. Due to its versatility and reliability, ELISA kits are essential tools in clinical laboratories, pharmaceutical development, and academic research.