Used in biomedical research to detect and quantify AP-1 transcription factor activity in cell lysates or tissue samples. Helps study cellular responses to stimuli such as stress, cytokines, growth factors, and UV radiation. Widely applied in cancer research, immunology, and drug development to assess signaling pathway activation, particularly the MAPK pathway. Enables high-throughput screening of compounds that modulate AP-1 activity, supporting the discovery of anti-inflammatory and anti-cancer agents. Provides a reliable, sensitive method for monitoring changes in gene regulation and cellular activation states.