Used in biomedical research to quantify levels of vascular endothelial growth factor A (VEGF-A) in biological samples such as serum, plasma, cell culture supernatants, and tissue extracts. This kit is essential for studying angiogenesis, the formation of new blood vessels, which plays a critical role in physiological processes like wound healing and pathological conditions including cancer, diabetic retinopathy, and age-related macular degeneration. Researchers use this assay to evaluate the effects of drugs, growth factors, or gene manipulations on VEGF-A expression. It is also applied in clinical research to identify biomarkers associated with tumor progression, inflammation, and cardiovascular diseases. The high sensitivity and specificity of the ELISA format allow for accurate measurement of VEGF-A concentrations in small sample volumes, supporting both basic science and translational studies.