Used in biomedical research to detect and quantify phosphorylated AF proteins in human samples, this ELISA kit supports studies on cell signaling pathways, particularly those involved in gene regulation and transcriptional activation. It is commonly applied in cancer research and immunology to monitor protein phosphorylation status, which can indicate activation or suppression of key regulatory proteins. The kit enables high-throughput analysis in clinical labs and pharmaceutical development for assessing response to therapeutic agents targeting kinase pathways.