Used in biomedical research to quantify soluble adenylate cyclase (sAC) levels in cell lysates, tissue extracts, and biological fluids. Enables investigation of sAC involvement in cellular signaling pathways, particularly those regulated by bicarbonate and calcium ions. Widely applied in studies related to sperm physiology, pH sensing, mitochondrial function, and metabolic regulation. Supports research into diseases linked to sAC dysfunction, including infertility, glaucoma, and certain neurological disorders. Provides a reliable, high-throughput method for monitoring sAC expression and activity in experimental models and drug development.