Used in research settings to quantify levels of MUC5AC, a gel-forming mucin, in biological samples such as cell culture supernatants, bronchoalveolar lavage fluid, and tissue homogenates. This ELISA kit enables scientists to study mucin overproduction in respiratory diseases like asthma, chronic obstructive pulmonary disease (COPD), and cystic fibrosis. It is also applied in investigating the role of MUC5AC in gastric protection, dry eye conditions, and certain cancers, particularly those involving mucosal tissues. The assay supports drug development and evaluation of anti-inflammatory or mucoregulatory therapies by providing a reliable measure of MUC5AC expression under various experimental conditions.