Used in research settings to detect and quantify rat cardanol (ANF) levels in biological samples, this ELISA kit enables studies on neuroendocrine responses, particularly related to atrial natriuretic factor activity in cardiovascular and renal function. It supports investigation into hypertension, heart failure, and fluid balance regulation by providing a sensitive and specific measurement tool for ANF concentration in rat serum, plasma, or tissue extracts. The assay is valuable in preclinical studies and drug development where monitoring hormonal changes is critical.