Used in immunological research to quantify Rat macrophage replacement activation-related chemical factor 1 (AmAC-1) levels in biological samples such as serum, plasma, or cell culture supernatants. The ELISA kit enables researchers to study macrophage activation states in inflammatory diseases, immune responses, and tissue repair processes in rat models. It is particularly useful in preclinical studies investigating autoimmune conditions, infections, and cancer-related inflammation where macrophage polarization plays a critical role. The assay provides high sensitivity and specificity for detecting subtle changes in AmAC-1 concentration, supporting data collection in mechanistic studies and therapeutic evaluations.