Used in biomedical research to measure cyclic adenosine monophosphate (cAMP) levels in rat samples. This assay helps study cellular signaling pathways, especially those involving hormones and neurotransmitters that activate G-protein-coupled receptors. Commonly applied in endocrinology, neuroscience, and pharmacology to evaluate drug effects, hormone activity, and intracellular responses in rat models. Enables high-throughput analysis of cAMP in tissue homogenates, cell lysates, and biological fluids with high sensitivity and specificity.