Used in biomedical research to quantify levels of butyryl coenzyme A and acetyl coenzyme A transferase enzymes in rat samples. Helps in studying metabolic pathways related to short-chain fatty acid metabolism, particularly in the liver and gastrointestinal tract. Supports research on mitochondrial function, energy metabolism, and disorders linked to metabolic dysregulation. Commonly applied in preclinical studies involving rodent models of diet-induced metabolic disease, gut microbiota effects, and neurodegenerative conditions where acetyl-CoA homeostasis plays a role. Provides a reliable, sensitive method for enzyme detection in tissue homogenates and biological fluids.