Used in biomedical research to detect and quantify levels of azidothymidine (AZT) in biological samples. Widely applied in pharmacokinetic studies to monitor drug concentration in experimental models, especially in HIV/AIDS research where AZT is used as an antiretroviral agent. The ELISA format allows for high sensitivity and specificity, enabling researchers to assess drug metabolism, distribution, and efficacy in laboratory animals. Also useful in toxicology studies to evaluate potential side effects of AZT exposure in rat models. Supports development and validation of dosing regimens in preclinical trials.