Used in immunological research to quantify soluble ALCAM levels in biological samples such as serum, plasma, or cell culture supernatants. Helps investigate immune cell activation and migration in inflammatory conditions, autoimmune diseases, and cancer progression. Commonly applied in studies involving leukocyte trafficking, endothelial cell interactions, and biomarker discovery. Provides a reliable, sensitive method for detecting ALCAM protein expression changes in experimental models and clinical research settings.