Used in biomedical research to quantitatively measure levels of 3-hydroxy-3-methylglutaryl coenzyme A synthase (HMGCS) in rat samples. This kit is particularly useful in studies related to cholesterol biosynthesis, ketogenesis, and mitochondrial metabolism. Researchers apply it in metabolic disease models, such as diabetes or fatty liver disease, to assess changes in HMGCS expression. It supports investigation into drug effects on metabolic pathways and helps evaluate the regulation of the mevalonate pathway in experimental conditions. The assay enables high-throughput analysis with high specificity and sensitivity, making it suitable for preclinical studies involving rat models.