Used in biomedical research to quantify levels of vascular endothelial growth factor B (VEGF-B) in mouse samples, particularly in studies related to angiogenesis, cardiovascular diseases, and metabolic disorders. The ELISA kit enables researchers to measure VEGF-B protein concentration in cell culture supernatants, serum, plasma, and tissue homogenates with high specificity and sensitivity. It is widely applied in preclinical studies investigating the role of VEGF-B in blood vessel formation, heart function, and insulin sensitivity. The assay supports the development of therapeutic strategies targeting the VEGF-B signaling pathway in conditions such as ischemic heart disease, diabetic complications, and obesity-related metabolic dysfunction.