Used in biomedical research to detect and quantify HMGB-1 protein levels in biological samples such as serum, plasma, and cell culture supernatants. Enables study of inflammation, sepsis, autoimmune diseases, and cancer progression. Supports investigation of HMGB-1's role as a damage-associated molecular pattern (DAMP) molecule in immune responses. Facilitates drug development and evaluation of therapeutic agents targeting inflammatory pathways. Commonly applied in preclinical research and clinical laboratories for biomarker analysis.