Used in immunological research to quantify interleukin-12B levels in biological samples such as serum, plasma, or cell culture supernatants. Enables study of immune responses, particularly in relation to inflammation, autoimmune diseases, and cancer immunotherapy. Commonly applied in preclinical studies to assess cytokine production in mouse models, supporting evaluation of therapeutic interventions and vaccine development. Provides high sensitivity and specificity for accurate detection of IL-12B protein expression.