Used in immunological research to quantify Granzyme B levels in biological samples such as serum, plasma, and cell culture supernatants. Enables assessment of cytotoxic T-cell and natural killer (NK) cell activity, which is critical in studying immune responses, autoimmune diseases, viral infections, and cancer immunotherapy. Widely applied in clinical research to monitor disease progression and therapeutic efficacy where immune cell activation plays a key role. The kit provides a reliable, sensitive, and high-throughput method for detecting Granzyme B, supporting studies in inflammation, transplantation, and immune monitoring.