Used in research to quantify NF-κB p65 protein levels in cell lysates or tissue extracts. Helps study inflammatory responses, immune regulation, and apoptosis. Commonly applied in drug development to assess compound effects on NF-κB signaling pathways. Enables high-throughput screening in disease models such as cancer, arthritis, and neurodegenerative disorders. Provides a reliable method for monitoring activation of transcription factors involved in cellular stress and inflammation.