Used in biomedical research to detect and quantify nuclear factor kappa B (NF-κB) activation in human cell and tissue samples. This kit enables researchers to study inflammatory responses, immune regulation, cell survival, and apoptosis by measuring levels of NF-κB proteins in nuclear extracts. It is widely applied in studies related to cancer, autoimmune diseases, and viral infections where NF-κB signaling plays a key role. The assay supports high-throughput screening of potential therapeutic compounds that modulate NF-κB pathways, making it essential in drug development and molecular biology labs.