Used in research settings to quantify β-trace protein (BTP) levels in human biological samples, primarily cerebrospinal fluid and serum. Enables studies on neurological disorders such as Alzheimer's disease, multiple sclerosis, and stroke, where BTP levels may serve as a biomarker for blood-brain barrier integrity and neuronal damage. Also applied in renal function research, as BTP is an endogenous marker for glomerular filtration rate, offering an alternative to creatinine. The ELISA format allows for high sensitivity and specificity, making it suitable for clinical and experimental investigations requiring precise protein measurement.