Used in biomedical research to quantify apolipoprotein B100 levels in rat serum or plasma samples. Enables assessment of lipid metabolism and cardiovascular disease risk in experimental animal models. Commonly applied in studies involving atherosclerosis, hyperlipidemia, and metabolic syndrome. Supports evaluation of drug efficacy targeting cholesterol regulation and lipoprotein transport. Provides a reliable, sensitive, and specific method for detecting apo-B100 in preclinical research settings.